anti irf3 (Cell Signaling Technology Inc)
Structured Review
Anti Irf3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/irf+3/IRF-3+XP+Rabbit+mAb/pmc13011919-263-28-30
Average 96 stars, based on 484 article reviews
Images
Related Articles
other:Article Title: The lysosomal LAMTOR-Rag complex functions as a checkpoint for antiviral interferon production Article Snippet: Membrane:Article Title: Enhanced B cell electroporation efficiency via inhibition of DNA-induced apoptosis and pyroptosis with pan-caspase inhibitor. Article Snippet: .. The PVDF membrane was incubated with the following primary antibodies: PARP (46D11) (9532, 1:1000; CST), GSDMD (ab209845, 1:1000; Abcam), Article Title: Microbial extracellular vesicles from min pigs remodel macrophage polarization via STING to sustain intestinal immune homeostasis Article Snippet: Next, the gel was prepared for Western blot analysis according to the SDS‒PAGE gel preparation kit (Beyotime P0012A China), and the proteins were transferred to a polyvinylidene fluoride membrane activated with methanol (Beyotime P0965 China) using transfer buffer (0.025 M Tris, 0.192 M glycine, and 20% methanol) after electrophoresis. .. The polyvinylidene fluoride membrane was blocked in PBST solution containing 0.01% Tween 20 and 5% skim milk at room temperature for 1 h. For detection of STING (Cell Signaling 13647 America), TBK 1 (Cell Signaling 3504 America), and Article Title: Microbial extracellular vesicles from min pigs remodel macrophage polarization via STING to sustain intestinal immune homeostasis. Article Snippet: Next, the gel was prepared for Western blot analysis according to the SDS‒PAGE gel preparation kit (Beyotime P0012A China), and the proteins were transferred to a polyvinylidene fluoride membrane activated with methanol (Beyotime P0965 China) using transfer buffer (0.025 M Tris, 0.192 M glycine, and 20% methanol) after electrophoresis. .. The polyvinylidene fluoride membrane was blocked in PBST solution containing 0.01% Tween 20 and 5% skim milk at room temperature for 1 h. For detection of STING (Cell Signaling 13647 America), TBK 1 (Cell Signaling 3504 America), and Incubation:Article Title: Enhanced B cell electroporation efficiency via inhibition of DNA-induced apoptosis and pyroptosis with pan-caspase inhibitor. Article Snippet: .. The PVDF membrane was incubated with the following primary antibodies: PARP (46D11) (9532, 1:1000; CST), GSDMD (ab209845, 1:1000; Abcam), Article Title: Microbial extracellular vesicles from min pigs remodel macrophage polarization via STING to sustain intestinal immune homeostasis Article Snippet: Next, the gel was prepared for Western blot analysis according to the SDS‒PAGE gel preparation kit (Beyotime P0012A China), and the proteins were transferred to a polyvinylidene fluoride membrane activated with methanol (Beyotime P0965 China) using transfer buffer (0.025 M Tris, 0.192 M glycine, and 20% methanol) after electrophoresis. .. The polyvinylidene fluoride membrane was blocked in PBST solution containing 0.01% Tween 20 and 5% skim milk at room temperature for 1 h. For detection of STING (Cell Signaling 13647 America), TBK 1 (Cell Signaling 3504 America), and Article Title: Microbial extracellular vesicles from min pigs remodel macrophage polarization via STING to sustain intestinal immune homeostasis. Article Snippet: Next, the gel was prepared for Western blot analysis according to the SDS‒PAGE gel preparation kit (Beyotime P0012A China), and the proteins were transferred to a polyvinylidene fluoride membrane activated with methanol (Beyotime P0965 China) using transfer buffer (0.025 M Tris, 0.192 M glycine, and 20% methanol) after electrophoresis. .. The polyvinylidene fluoride membrane was blocked in PBST solution containing 0.01% Tween 20 and 5% skim milk at room temperature for 1 h. For detection of STING (Cell Signaling 13647 America), TBK 1 (Cell Signaling 3504 America), and Article Title: cGAS-IFN-I responses by extracting nuclear DNA from dying cells via nucleocytosis Article Snippet: Proteins were resolved on the NuPAGE 4–12% Bis-Tris Gel (Thermo Fisher Scientific), and electrotransferred to nitrocellulose membranes included in the iBlot Transfer Stack using the iBlot Dry Blotting System (Thermo Fisher Scientific). .. The membranes were blocked in 5% bovine serum albumin (BSA) in PBS with 0.1% Tween 20 and then incubated with primary antibodies against GAPDH (14C10; Cell Signalling Technology [CST], Danvers, MA, USA), |

